الرئيسية / الفعاليات العلمية / ابحاث / Cloning and Expression of GST-HMPREF0351_11084 Gene in JM105 Cells: A New Potential Protein Vaccine for Pneumococcal

Cloning and Expression of GST-HMPREF0351_11084 Gene in JM105 Cells: A New Potential Protein Vaccine for Pneumococcal

Sddiq Ghani Al-Muhanna1, 2*, Abbas Shakir Jawad1
1Department of Biology, Faculty of Science, University of Kufa, Najaf, Iraq
2Al-Kafeel University College, Department of Pathological Analysis Techniques, Najaf, Iraq Kufa, P.O.
Box (21), An Najaf Governorate, Iraq 31003.
*Corresponding Author: Sddiq Ghani Al-Muhanna
Abstract
The HMPREF0351_11084 gene encoding pneumococcal vaccine antigen A family protein (GeneID:
12999770) was optimized and synthesized, then sub-cloned into pGH cloning vector. The amplified
HMPREF0351_11084 gene was incorporated into
Bam HI and Eco RI sites of pGEX-2T GST expression
vector under the control of tac promoter. The recombinant pGEX-2T was transformed and expressed into
JM105 cells. The protein of expressing bacteria was analyzed by SDS-PAGE and the target protein was
detected by Western blot – enhanced chemiluminescence (WB-ECL) assay. The result shows predicted
target fusion protein with molecular weight 51.2 kDa. The current study describes for the first time the
expression of HMPREF0351_11084 gene, and so attempts to produce a new potential protein vaccine for
pneumococcal. Further extensive study is required to generate more data about HMPREF0351_11084
protein and evaluate its immunization in elicits antibodies that are capable of passively protecting
against pneumococcal infections.
Keywords: Fusion protein, PGEX-2T, HMPREF0351_11084, ECL, Pneumococcal vaccine.

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