الرئيسية / الفعاليات العلمية / ابحاث / Amylase production , purification and characterization from Escherichia coli

Amylase production , purification and characterization from Escherichia coli

Abstract
This study was conducted in the laboratories of Biology Department, faculty of Science, which deal with isolation, purification and characterization of of amylase by Escherichia coli which carried out for enhanced production of amylase using starch (1%) as the substrate of enzyme , the production was carried out by submerged fermentation , the best conditions were the isolated of amylase in synthetic medium , it gave high titer of amylase activity , the ammonium sulfate as nitrogen source , incubation period 48 h , the starch as carbon source, incubation temperature 30 °C and pH = 7 , The amylase was purified using precipitation by ammonium sulphate (60%) and dialysis , the refined amylase had a maximum activity at pH = 7, the amylase was stable with pH values ranging between (7 – 8) and in temperature 30 °C also amylase was stable in (30- 40 ) °C analyses of the amylase for molecular weight was carried out by SDS-PAGE electrophoresis which revealed 52 KDa.

1* Baydaa A. Hassan

1* Nibras yahya alsalami

*2Mohammed A. Jebor

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