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Cytotoxic Activity of Alkaloids Extracted from Trigonella foenum graecum (Fenugreek) against Breast Cancer Cell Line

1Alaauldeen S.M.AL-Sallami ; 2Zuhair Salih Al-Labban; Sanaa mohemmed ali
1,2 Iraq, 1,2 University of Kufa /1 Faculty of Science / 2 Faculty of Medicine
Abstract
The present study was investigated to Screening for cytotoxic activity of total alkaloid extracts of Trigonella foenum graecum (Fenugreek) against breast cancer cell line Ahmed-Mohammed-Nahi,2003 (AMN-3), AMJ-13 and normal cell line Rat Embryo Fibroblast (REF). The Trigonella foenum powders were extracted separately with 80% Ethanol and total alkaloids separated at pH 2 and 10 with HCl and ammonia . Total alkaloids were detected qualitatively by Mayer’s, Hager‘s , and Dragendorff’s reagents. The cytotoxic activity was evaluated by crystal violet assay. The results show that The extract of Trigonella foenum graecum had total alkaloid in extract about 1.7% resulting from getting 4.4 gram of dry alkaloid extract from 252 gram of dry powder of Trigonella foenum – graecum. The alkaloids reduced the cell viability of all cell lines AMN-3,AMJ-13 and REF, and the inhibitory concentration 50% of cells were 1029 μg/ml, 1069 μg/ml and 1724 μg/ml for AMN-3, AMJ13 and REF respectively. Trigonella foenum alkaloids showed variable cytotoxic activity against cancer and normal cell lines depending on the alkaloid concentrations and cell line types.
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