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GENETIC RELATIONSHIP AMONG TEN WHEAT GENOTYPES USING SEVENTEEN RAPD MARKERS

Melath Kadem Farhood AL-Ghufaili and Attyaf Jameel Thamir Al-Tamimi*
Faculty of Science, University of Kufa, Iraq.
Abstract
This study was conducted for determination DNA fingerprint and estimation of genetic diversity among ten wheat (Triticum
aestivum L.) genotypes using seventeen RAPD Markers. Primers OPB-06, OPC-05, OPH-01 and UBC-126 gave unique
fingerprint for studied genotypes. Primer OPG-09 gave higher value for polymorphism . The higher efficiency and discriminatory
value was produced by primer UBC-126. High genetic distance was 0.546 between Buhuth22 and Faris while low genetic
distance was 0.142 between Rasheed and Iraq. Cluster analysis (Phylogenetic tree) by UPGMA based dendrogram revealed
that studied genotypes grouped in two main clusters. Low polmorphisim (36.827) revealed by studied primers. Results show
that RAPD markers could efficiently reveal genetic variation and fingerprint wheat genotypes .

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