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Isolation and Diagnosis of Fungi Which Producing Aflatoxin B1in Chips in Local and Imported Markets Najaf Province

Journal of Global Pharma Technology
Available Online at: www.jgpt.co.in

S.W. Kadium1, R. A. Sahib*1
1Department of Biology,College of Science /University of Kufa,Najaf- Iraq.
*Corresponding Author Email: [email protected]; [email protected]
Abstract
This study included isolating and diagnosing contaminated fungi from some local and imported chipsin
Najaf Province,and to detect their ability to produce aflatoxin B1. The results showed that highest
percentageof visible fungi namely are Aspergillusflavus, A. niger, A. glaucus, Alterneria sp., Fusarium sp.
and A. terreusith ratio are (100, 95, 92,80, 75, 5.7) % respectively in Wejoh chips and these fungi
appeared in all types of chips under study. The results of the frequency of the same fungi in the wejoh
chips are (80,70, 68, 52, 50,2) % respectively.A.flavus is more visible and frequently in thesame types of
chips,and preferable sabouraud dextrose (agar,broth) medium to growth. Primary result proved that A.
flavus producing aflatoxin B1in Thinlayerchromatography (TLC) which appear blue spot of aflatoxin B1
with rate of flow (RF) is 56 % under UV light expose with wavelength of (365 nm).High performance
liquid chromatography (HPLC) results show that retention time or major peak of standard aflatoxin B1
located at 10.54 minutes, while a filter extract of A. flavusis10.75 which identifies with aflatoxin B1
standardwhich located in the same area

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