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Antibacterial Activity of Mono and Bimetallic Au:Ag Colloidal Nanoparticles Prepared by Pulse Laser ablation PLA

Adel H. Omran Alkhayatt1
Mahdi Husain Moheel2
Mohammad Malik Abood1
University of Kufa, Faclty of Sciences, 1
Physics Department,
2Biology Department, Najaf,
adilh.alkhayat@uokufa.edu.iq mahdialammar@yahoo.com
Au, Ag mono and Bimetallic Au:Ag colloidal nanoparticles were synthesized in two
steps using pulsed laser ablation in liquid technique (pure Au, Ag metal plates of immersed in
deionized water). The pulse laser (Q-switched, Nd: YAG)1064 nm with energy 700 mJ,
frequency 5 Hz and 200 laser pulses at room temperature have been used. Absorbance (SPR
band), size, surface morphology and structure of the synthesized Nps have been studied and
investigated by UV-Vis spectrophotometer, transmission electron microscopy, and x-ray
diffraction. The absorbance spectrum of Au, Ag and Au:Ag nanoparticles shows sharp and
single peaks around 513 nm, 398 nm and 523 nm respectively. This gives indicate that the
production of pure and spherical Au and Ag NPs with average size in the range 16 – 100 nm.
The particles size measurements were confirmed by TEM. The Au:Ag nanoalloy appears to
be nearly spherical with an average size ranging from 29 to 72 nm. and the SPR band at (450
nm ). The XRD result showed very low crystallinity structure. The antibacterial activity of
Au, Ag and bimetallic Au:Ag NPs were evaluated for NPs obtained by PLAL using applied
pulses (100,150,200) in DI water, on the Gram-positive isolate (Staphylococcus aureus) and
Gram-negative isolate (Escherichia coli). The results and images of inhibition zones diameter
showed that the NPs have synergistic effects on the studied bacteria up to 15 mm and 4 mm at
the highest and lowest values and it increase with the increase of laser pulses number.
Keywords: Au, Ag mono and Bimetallic nanoparticles; pulsed laser ablation in liquid;
SPR band; antibacterial Activity.


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